Onkologie. 2013:7(2):74-77

Fluorescence in situ hybridization in diagnostics melanocytic lesions

Jana Dvořáčková^1,2,3, Jana Žmolíková^1,2, Jan Mužík⁴, Magdalena Uvírová^1,2

¹ Lékařská fakulta Ostravské univerzity v Ostravě

² CGB laboratoř a.s., Ostrava

³ Ústav patologie FN Ostrava

⁴ Institut biostatistiky a analýz, Masarykova univerzita, Brno

Melanocytic lesions exhibit significant morphological heterogenity, making their diagnosis difficult, although both sophisticated histopathological

and immunohistochemical methods are used. The introduction of molecular genetic method flourescence in situ hybridization

(FISH) has contributed significantly to the diagnosing of melanocytic lesions. Between 2008 and 2012, we studied a total of 160 samples

of different types of melanocytic lesions, including 44 nevi and 116 melanomas, using a four-color probe mix for CCND1, RREB1 and MYB

genes and for the centromere of chromosome 6. A positive result was found in 116 melenoma samples (72.5 %) and a negative result in

41 samples (25.6 %). A borderline value was found in two samples (1.2 %), and an atypical finding (monosomy of chromosome 6) was seen

in a melanoma recurrence in one case (0.6 %). Sensitivity and specificity were 96.6 % and 91 %, respectively. These results show that FISH

is a suitable and relatively low-cost method for accurate, rapid diagnosis of ambiguous melanocytic lesions. The speed and accuracy

of diagnosis of highly malignant melanomas are crucial and have great impact on the prognosis and success of a patient‘s treatment.

Keywords: melanoma, naevus, fluorescence in situ hybridization, FISH, gene

Published: May 1, 2013  Show citation